Liquid Plasma vs Thawed Plasma: Tracking Coagulation Factor Activity Changes During Storage

Area of Science:

• Transfusion Medicine
• Hematology
• Biochemistry

Background:

• Liquid plasma (LQP) presents a longer shelf-life alternative to thawed plasma (TP) for emergent transfusions.
• Assessing coagulation factor stability and activity in LQP is crucial for its clinical application.

Purpose of the Study:

• To measure fibrinogen, Protein C, Protein S, FV, FVII, and FVIII activity in LQP.
• To quantify changes in these factor levels in LQP during storage.
• To compare LQP factor activity with that of TP.

Main Methods:

• Coagulation factor activities were measured in LQP (n=26) at Days 15, 26, and 27, and in TP (n=24) at Day 5.
• Bayesian statistical modeling was employed to compare factor activities and assess storage-related changes.

Main Results:

• Fibrinogen and Protein C activity in LQP at Day 26 (LQP26) were comparable to TP at Day 5 (TP5).
• FV, FVII, and FVIII activities were slightly lower in LQP26 compared to TP5.
• Protein S activity was significantly lower in LQP26 (28.0%) than in TP5 (64.9%).
• FVII activity decreased in LQP from Day 15 to Day 26, while other factors remained stable.

Conclusions:

• LQP exhibits comparable fibrinogen, Protein C, FV, FVII, and FVIII activities to TP for emergency use, with the exception of lower Protein S levels.
• LQP is a viable alternative for emergency transfusions and massive transfusion protocols due to its extended shelf life and adequate factor levels.