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Related Experiment Video

Updated: Sep 9, 2025

Expanding the Toolkit for In Vivo Imaging of Axonal Transport
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A Toolkit for In Vivo Mapping and Modulating Neurotransmission at Single-Cell Resolution.

Andrea Cuentas-Condori1, Patricia Chanabá-López1, Matthew Thomas1

  • 1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine; New Haven, CT 06536, USA.

Biorxiv : the Preprint Server for Biology
|September 2, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed genetic tools in *Caenorhabditis elegans* to visualize and control neurotransmitter vesicles. This revealed that over 10% of neurons use co-transmission, advancing our understanding of synaptic communication.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Understanding neurotransmission requires tools to track and manipulate transmitter-specific vesicles *in vivo*.
  • Current methods lack the specificity and functionality for detailed synaptic analysis.

Purpose of the Study:

  • To develop a suite of genetic tools for fluorescently labeling and conditionally ablating vesicular transporters in *Caenorhabditis elegans*.
  • To map neurotransmitter co-expression and investigate vesicle trafficking dynamics.

Main Methods:

  • Engineered endogenously tagged vesicular transporters for glutamate, GABA, acetylcholine, and monoamines.
  • Developed conditional knockout strains for targeted disruption of neurotransmitter packaging.
  • Utilized a structure-guided approach based on protein topology and evolutionary conservation.

Main Results:

  • Successfully visualized and manipulated transporter function while maintaining physiological activity.
  • Mapped vesicular transporter co-expression, identifying co-transmission in over 10% of neurons.
  • Demonstrated partially distinct vesicle pools for serotonin and acetylcholine in ADF sensory neurons.

Conclusions:

  • The developed toolkit provides a powerful platform for *in vivo* mapping, monitoring, and manipulation of neurotransmitter identity and use.
  • The molecular strategies are generalizable across species for dissecting synaptic communication.