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Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Related Experiment Video

Updated: Sep 9, 2025

In vivo Dual Substrate Bioluminescent Imaging

In vivo Dual Substrate Bioluminescent Imaging

Published on: October 11, 2011

An open, integrated platform for multiplexed bioluminescence microscopy.

Lorenzo Scipioni, Belen Torrado, Giulia Tedeschi

Biorxiv : the Preprint Server for Biology

|September 2, 2025

Summary

This summary is machine-generated.

This study introduces a new imaging package for bioluminescence and fluorescence microscopy. The platform offers tools for experiment design, microscope upgrades, and data analysis, making advanced imaging more accessible.

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Area of Science:

Biophotonics and Imaging Technologies
Microscopy and Spectroscopic Techniques

Background:

Advanced microscopy techniques like bioluminescence and fluorescence imaging are crucial for biological research.
Current platforms can be expensive and complex, limiting accessibility.

Purpose of the Study:

To introduce a novel, integrated imaging package designed to democratize simultaneous bioluminescence and fluorescence microscopy.
To provide researchers with accessible tools for experimental design, hardware upgrades, and data analysis.

Main Methods:

Development of PhasorViewer for experimental design and probe selection.
Implementation of PhasorScope, an open-source, cost-effective microscopy framework.
Creation of PhasorAnalysis, a user-friendly pipeline for phasor imaging data quantification.

Main Results:

Demonstration of a comprehensive platform for all-in-one bioluminescence and fluorescence microscopy.
Successful multiplexed, simultaneous imaging using readily available optical reporters.
Validation of the platform's utility in simplifying complex imaging experiments.

Conclusions:

The developed imaging package significantly enhances accessibility to advanced microscopy.
The integrated tools facilitate efficient experimental design, hardware implementation, and data analysis.
This democratizes high-performance multiplexed imaging for a wider research community.