Selective IgM digestion improves anti-AAV IgG detection in the immune complex assay format
Uwe Wessels1, Alexander Pöhler1, Francesca Ros1
1Roche Pharma Research & Early Development (pRED), Pharmaceutical Sciences, Roche Innovation Center Munich, Roche Diagnostics GmbH, Penzberg, Germany.
Aim:
To confirm the observation of IgM interference in the anti-adeno-associated virus (AAV) IgG immune complex (IC) assay format and to verify that IgM-specific digestion can improve anti-AAV IgG detection in IC assays.
Methods:
Treatment-emergent anti-AAV2 and anti-AAV9 IgG signals were measured in IC assays with and without IgM-specific digestion. Anti-AAV2 and anti-AAV9 IgM signals were measured in parallel.
Results:
IgM digestion increased anti-AAV2 and anti-AAV9 IgG signals when anti-AAV2 or anti-AAV9 IgM were present in the matrix.
Conclusions:
Co-existing anti-AAV IgM cause interference in the anti-AAV IC assay format. Selective IgM digestion improves the detection of anti-AAV IgG in the IC assay.
