An open source for multiplexed, stable and transient flows to advance life sciences using microfluidic control...

Area of Science:

• Life Sciences
• Bioengineering
• Microfluidics

Background:

• Multiplexed fluid control is crucial but challenging in life sciences and bioengineering.
• Existing systems can be costly and lack flexibility for diverse applications.

Purpose of the Study:

• To present microfluidic sequence automation (MiSA), an open-source system for flexible, multiplexed fluid control.
• To demonstrate MiSA's capability for stable flow, pulsed flow, and various microfluidic applications.

Main Methods:

• MiSA integrates an Arduino Micro, ten solenoid valves, a pressure regulator, and a flow sensor for pressure-based feedback control.
• A potentiometer is used to tune pressure control for stable microflow, especially at low flow rates.
• The system was validated using hybridization-based in situ sequencing and extended for droplet generation, fiber spinning, and atomization.

Main Results:

• MiSA provides 10-plex capability for constant flow and pulsed flow with millisecond precision.
• The system demonstrated stable microflow control under low flow resistance.
• Successful application in in situ sequencing, droplet generation, fiber spinning, and protein solution atomization.

Conclusions:

• MiSA offers a cost-effective, reliable, and flexible solution for multiplexed microfluidic control.
• The open-source nature of MiSA facilitates rapid exploration of microfluidic applications.
• This system empowers researchers with advanced fluidic capabilities for diverse scientific endeavors.