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Related Experiment Video

Updated: Sep 9, 2025

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

A single-nanoprobe-integrated multi-modal microscope (SNIM).

Yang Xu¹, Chen Zhang¹, Yunze Zhou¹

¹Laboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang University, 310058 Hangzhou, China.

The Review of Scientific Instruments

|September 3, 2025

Summary

This summary is machine-generated.

Researchers developed a new nanoprobe for precise intracellular measurements using manipulable single nanodiamonds (MSNs). This quantum sensing tool enables active control and multi-physics field analysis within live cells.

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Area of Science:

Quantum sensing
Biophysics
Nanotechnology

Background:

Nitrogen-vacancy (NV) centers in nanodiamonds offer potential for intracellular sensing.
Current limitations include the lack of active control for targeted intracellular measurements.

Purpose of the Study:

To develop an integrated nanoprobe system for precise, active manipulation and multi-physics field measurement within live cells.
To overcome the challenge of controlling nanodiamond probes inside cells for targeted sensing.

Main Methods:

Utilized a manipulable single nanodiamond (MSN) approach integrated with a nanopipette system for high-precision manipulation.
Developed a multi-modal microscope capable of live cell culture, in situ observation, and local field modulation via electric and optical signals.
Employed a multi-stack sample cell with a vibration-free thermostat for long-term live cell experiments.

Main Results:

Demonstrated active manipulation and precise positioning of the nanoprobe within a cellular environment.
Achieved real-time, in situ intracellular multi-physics field measurements.
Validated the system's capability for long-term live cell incubation and observation.

Conclusions:

The integrated nanoprobe system successfully combines manipulation and measurement capabilities for live cell studies.
This quantum sensing tool provides optimal conditions for investigating complex intracellular dynamics.
The developed technology offers a powerful new approach for intracellular research.