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Updated: Sep 9, 2025

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
Hsa_circ_0005325 Regulates the Proliferation, Apoptosis, Colony Formation, Migration, and Angiogenesis-Promoting
Zhihan Lin1, Yating Fu2, Lei Mao1
1Department of Stomatology, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.
Objective:
To explore the mechanism by which hsa_circ_0005325 affects the proliferation, apoptosis, colony formation, migration, and angiogenesis-promoting behavior of oral squamous cell carcinoma cells through the miR-433-3p/HMGA2 axis.
Material And Methods:
qRT‒PCR was used to measure the expression of hsa_circ_0005325 in SCC25 and CAL-27 cells and normal human oral epithelial cells (HOK). SCC25 and CAL-27 cells were cultured, and Cell Counting Kit-8 (CCK-8), cell apoptosis, plate colony formation, Transwell migration and a tube formation assays were used to detect changes in cell proliferation, apoptosis, colony formation, migration and angiogenesis, respectively.
Results:
The expression of hsa_circ_0005325 was significantly increased in SCC25 and CAL-27 cells. Compared with those in the sh-NC group, the percentages of apoptotic SCC25 and CAL-27 cells in the sh-circ_0005325 group were significantly greater, and their proliferation, colony formation, migration and angiogenesis capacities were significantly lower (p < 0.05). Moreover, the protein expression level of HMGA2 was significantly decreased, and the expression level of miR-433-3p was significantly increased in the sh-circ_0005325 group versus the control group (p < 0.05).
Conclusion:
Hsa_circ_0005325 is highly expressed in SCC25 and CAL-27 cells. Downregulation of hsa_circ_0005325 can inhibit the proliferation and invasion of SCC25 and CAL-27 cells and promote their apoptosis.
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