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Related Experiment Video

Updated: Sep 9, 2025

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

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Published on: January 15, 2020

Development Strategies for Influenza Vaccines Utilizing Phage RNA Polymerase and Capping Enzyme NP868R.

Weijun Wang¹, Zihan Ma², Qiuli Lou¹

¹Center for Cell and Gene Circuit Design, CAS Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Chem & Bio Engineering

|September 3, 2025

Summary

Novel DNA and RNA vaccine platforms show promise for influenza prevention. These advanced vaccines utilize phage RNA polymerase and improved production methods, demonstrating effective immune responses in animal studies for better disease control.

Keywords:

capping enzyme influenza vaccine phage RNA polymerase positive feedback transcription rdVSV

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Area of Science:

Vaccinology
Molecular Biology
Immunology

Background:

Influenza poses significant global health and economic challenges.
Traditional influenza vaccines have limitations in efficacy against new strains.
Novel vaccine strategies are urgently needed to address influenza threats.

Purpose of the Study:

To explore and develop advanced DNA and RNA vaccine platforms for influenza.
To enhance antigen expression and immune response using innovative molecular tools.
To improve vaccine production efficiency and safety.

Main Methods:

Developed a phage RNA polymerase-dependent DNA vaccine for high-efficiency hemagglutinin (HA) antigen expression.
Engineered a replication-deficient vesicular stomatitis virus (rdVSV) expressing HA as a self-amplifying RNA vaccine.
Utilized T7 RNA polymerase fused to a capping enzyme for improved rdVSV rescue and production.

Main Results:

DNA vaccine strategy achieved high-efficiency cytoplasmic antigen expression, reducing genomic integration risks.
RNA vaccine platform yielded high titers (1.2 × 10^7 PFU/mL) with faster production and improved safety.
Animal studies showed enhanced HA protein expression, higher antibody titers, and robust humoral and cellular immune responses.

Conclusions:

Both DNA and RNA vaccine platforms demonstrate significant potential for influenza control.
These platforms offer versatile tools for rapid response to emerging pathogens.
Further optimization and clinical validation could lead to new solutions for infectious disease prevention.