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ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
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Biosensors-based polypyrrole/metal-organic framework core-shell for mycotoxins detection.

Mostafa Fytory1, Fatma Besbes2, Diana Dragoe3

  • 1Université Paris-Saclay, Institut de Chimie Moléculaire et des Matériaux d'Orsay (ICMMO) UMR-CNRS Orsay, 17 avenue des sciences, 91405, Orsay, France; Material Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, 62511, Beni-Suef, Egypt.

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|September 3, 2025
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Summary

A novel biosensor using polypyrrole and a zirconium metal-organic framework detects ultra-trace levels of the carcinogenic mycotoxin Ochratoxin A (OTA) in food. This sensitive and selective platform offers rapid detection without sample pretreatment, enabling safer food consumption.

Keywords:
AptamerBiosensorsElectrochemicalOchratoxin APolypyrrole (Ppy)Zr-NMOF

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Area of Science:

  • * Materials Science: Development of novel core-shell nanostructures for advanced sensor applications.
  • * Analytical Chemistry: Electrochemical detection of mycotoxins in complex matrices.

Background:

  • * Ochratoxin A (OTA) is a prevalent, carcinogenic mycotoxin found in food and beverages, posing significant health risks.
  • * Current detection methods like HPLC are cumbersome, while electrochemical aptasensors require complex fabrication and offer limited output.
  • * A need exists for sensitive, selective, rapid, and scalable biosensing platforms for mycotoxin detection.

Purpose of the Study:

  • * To develop a highly sensitive and selective electrochemical biosensor for ultra-trace Ochratoxin A detection.
  • * To create a stable, core-shell nanostructure-based platform for simplified mycotoxin analysis.
  • * To enable direct detection of OTA in food matrices without prior sample treatment.

Main Methods:

  • * Fabrication of a core-shell nanostructure using conductive polypyrrole (Ppy) and Zirconium nanosized metal-organic framework (Zr-NMOF).
  • * Immobilization of DNA aptamers onto the positively charged Ppy/Zr-NMOF nanostructure via electrostatic interaction.
  • * Electrochemical detection of OTA based on the synergistic effect of Ppy and Zr-NMOF on electrical properties.

Main Results:

  • * Demonstrated ultra-trace detection of OTA with a limit of detection of 0.24 pM (0.1 ng/L).
  • * Achieved high selectivity for OTA over Ochratoxin B (OTB) and a wide dynamic linear range.
  • * Successfully detected OTA directly in red wine with high recovery, comparable to standard methods.

Conclusions:

  • * Presented the first Ppy/Zr-NMOF/DNA biosensor with enhanced electrical and stable properties for mycotoxin detection.
  • * The developed bioelectronic interface allows sensitive detection of mycotoxins at concentrations as low as 0.1 ng/L.
  • * This innovative approach is adaptable for detecting various contaminants by modifying aptamers, offering a versatile platform for food safety.