Jove
Visualize
Contact Us

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.6K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.6K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

6.6K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Genetically Encoded Tools to Monitor and Interrogate Membrane Contact Sites.

Contact (Thousand Oaks (Ventura County, Calif.))·2026
Same journal

ER-driven Contact Sites in Autophagosome Biogenesis Sequence.

Contact (Thousand Oaks (Ventura County, Calif.))·2026
Same journal

mtDNA-Depleted Mitochondria Form Sites of Contact with the Nucleus and Alter the Cellular Epigenome.

Contact (Thousand Oaks (Ventura County, Calif.))·2026
Same journal

A Novel Phloem-Specific HVA22-Like Protein Facilitates Protein Movement via Plasmodesmata in Potato.

Contact (Thousand Oaks (Ventura County, Calif.))·2026
Same journal

Corrigendum to "Remodelling of Cellular Protein Homeostasis by Enhanced ER-Mitochondrial Tethering".

Contact (Thousand Oaks (Ventura County, Calif.))·2026
Same journal

What Peroxisomes (Don't) do to Mitochondria.

Contact (Thousand Oaks (Ventura County, Calif.))·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 9, 2025

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
11:53

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Published on: March 31, 2023

1.2K

MCS Ultrastructural Analyses Using Electron Microscopy.

Atsuki Nara1

  • 1Department of Biological Data Science, Nagahama Institute of Bioscience and Technology, Nagahama-city, Japan.

Contact (Thousand Oaks (Ventura County, Calif.))
|September 4, 2025
PubMed
Summary
This summary is machine-generated.

Membrane contact sites (MCSs) are crucial cellular junctions for organelle communication and material exchange. Electron microscopy (EM) is key to understanding their structure and function in cell biology.

Keywords:
ERautophagosomecontactelectron microscopyendosomemembrane contact sitesmitochondrianucleusplasma membranetether

More Related Videos

Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
10:52

Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques

Published on: June 1, 2016

11.6K
Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
08:46

Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells

Published on: June 23, 2023

1.8K

Related Experiment Videos

Last Updated: Sep 9, 2025

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
11:53

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Published on: March 31, 2023

1.2K
Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
10:52

Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques

Published on: June 1, 2016

11.6K
Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
08:46

Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells

Published on: June 23, 2023

1.8K

Area of Science:

  • Cell Biology
  • Organelle Biology
  • Biochemistry

Background:

  • Membrane contact sites (MCSs) are specialized microdomains facilitating inter-organelle communication.
  • They are vital for lipid and ion exchange, metabolite transport, and intracellular signaling.
  • Understanding MCSs is critical for comprehending cellular metabolic regulation and function.

Purpose of the Study:

  • To review the ultrastructure of membrane contact sites.
  • To elucidate the role of electron microscopy (EM) in studying MCSs.
  • To highlight the physiological significance of MCSs in cell biology.

Main Methods:

  • Review of existing literature on MCS ultrastructure.
  • Focus on the application of electron microscopy (EM) techniques.
  • Analysis of how EM reveals MCS structure and function.

Main Results:

  • MCSs are essential for localized substance exchange between organelles.
  • EM provides critical structural details necessary for understanding MCS function.
  • The review synthesizes current knowledge on MCS ultrastructure and EM applications.

Conclusions:

  • MCS structure is fundamental to their role in cellular processes.
  • Electron microscopy is indispensable for detailed MCS analysis.
  • Further research into MCSs will advance our understanding of cell biology and metabolism.