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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Sensitive Bioassay with an Ultralarge Dynamic Range via Microlaser Ensemble Quenching.

Weishu Wu1,2,3, Yuhang Cao1,2,3, Xiaotian Tan4

  • 1Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States.

ACS Sensors
|September 4, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microlaser ensemble (ME) bioassay for precise analyte detection. The platform uses lasing threshold distribution to quantify concentrations, achieving ultra-low detection limits and a wide dynamic range.

Keywords:
ELISAdigital bioassaymicrolasermicrolaser ensemblemicrolaser quenching

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Area of Science:

  • Biophotonics
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Microlaser ensembles (ME) offer potential for sensitive bioassays.
  • Lasing properties are sensitive to surface modifications and molecular interactions.
  • Existing methods may lack the sensitivity or dynamic range required for certain applications.

Purpose of the Study:

  • To develop and validate a novel bioassay platform utilizing microlaser ensemble (ME) lasing threshold distribution.
  • To establish a method for measuring analyte concentrations in solution based on quenching effects in MEs.
  • To demonstrate the platform's capability for high sensitivity and broad dynamic range detection.

Main Methods:

  • Formation of a microlaser ensemble (ME) using dye-doped microbeads in a micro Fabry-Perot cavity.
  • Surface modification of microbeads with biorecognition molecules for analyte capture.
  • Measurement of lasing threshold distribution across the ME under varying analyte concentrations.
  • Application of a theoretical model to correlate lasing threshold distribution with analyte concentration.

Main Results:

  • The microlaser ensemble (ME) exhibits a measurable lasing threshold distribution directly related to analyte concentration.
  • A detection limit of 0.1 pg/mL was achieved for streptavidin.
  • The platform demonstrated a dynamic range exceeding 5 orders of magnitude.

Conclusions:

  • The microlaser ensemble (ME) quenching method provides a sensitive and robust platform for bioanalysis.
  • This approach enables low detection limits and superior dynamic range, outperforming traditional methods.
  • The developed bioassay platform holds promise for various diagnostic and research applications.