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Bright Thermo-resilient and Promiscuous Zombie Protein for Lighting Applications.

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Researchers developed a highly stable fluorescent protein from extremophiles for sustainable lighting. This protein, SPritZ and its enhanced variant eSPritZ, improves biohybrid light-emitting diodes (BioHLEDs) for better performance and durability.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Biophysics

Background:

  • Proteins are crucial for advanced materials, including optical and electrical applications.
  • Biohybrid light-emitting diodes (BioHLEDs) offer sustainable alternatives but require robust light-emitting components.
  • Fluorescent proteins (FPs) from extremophiles are potential candidates for demanding BioHLED applications due to their inherent stability.

Purpose of the Study:

  • To identify and engineer a highly stable fluorescent protein for BioHLEDs.
  • To explore the potential of extremophile FPs for tunable light emission and enhanced resilience.
  • To improve the stability and performance of BioHLEDs using engineered proteins.

Main Methods:

  • Phylogenetic analysis of 182 phycobiliproteins from 29 thermophiles to identify ancestral traits.
  • Heterologous expression of a thermostable phycobiliprotein (SPritZ) in *Escherichia coli*.
  • Rational mutagenesis to create a enhanced, more resilient variant (eSPritZ) and BioHLED fabrication.

Main Results:

  • Identification of a versatile, ancestral phycobiliprotein with potential for tunable emission.
  • Successful expression and engineering of SPritZ into a brighter, more thermostable eSPritZ variant.
  • Fabrication of BioHLEDs demonstrating 2.5-fold enhanced stability using SPritZ and eSPritZ.

Conclusions:

  • Extremophile-derived fluorescent proteins offer a promising route to stable and tunable light emission for BioHLEDs.
  • Engineered proteins like eSPritZ significantly enhance the durability and performance of biohybrid devices.
  • This work paves the way for sustainable, protein-based lighting technologies.