Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

9.5K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.5K
Condensins02:15

Condensins

3.6K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.6K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.2K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.2K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.5K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.5K
Heterochromatin02:38

Heterochromatin

14.3K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
14.3K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.5K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutual antagonism between PRC1 condensates and SWI/SNF in chromatin regulation.

Molecular cell·2026
Same author

How the non-motile kinesin KIF7 adapts conserved kinesin principles for its function in Hedgehog signaling.

bioRxiv : the preprint server for biology·2026
Same author

Multiomic analysis of clonal development reveals new regulators of leukemic cell growth.

Genes & development·2026
Same author

A minimum module for positioning the Chromosomal Passenger Complex at the cell center for cytokinesis.

bioRxiv : the preprint server for biology·2025
Same author

Micron-scale protein transport along microtubules by kinesin-driven shepherding.

bioRxiv : the preprint server for biology·2025
Same author

WSTF nuclear autophagy regulates chronic but not acute inflammation.

Nature·2025

Related Experiment Video

Updated: Sep 9, 2025

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

6.9K

Mutual Antagonism Between PRC1 Condensates and SWI/SNF in Chromatin Regulation.

Stefan Niekamp1,2, Sharon K Marr1,2, Rebecca Sanon1,2

  • 1Department of Molecular Biology, Massachusetts General Hospital Research Institute, Massachusetts General Hospital, Boston, MA 02114, USA.

Biorxiv : the Preprint Server for Biology
|September 5, 2025
PubMed
Summary

Polycomb Repressive Complex 1 (PRC1) condensates exclude SWI/SNF from chromatin, revealing a mechanism for gene regulation. This antagonism balances competing activities crucial for development.

More Related Videos

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

9.9K
Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
11:06

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae

Published on: December 29, 2017

12.8K

Related Experiment Videos

Last Updated: Sep 9, 2025

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

6.9K
Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

9.9K
Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
11:06

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae

Published on: December 29, 2017

12.8K

Area of Science:

  • Chromatin Biology
  • Gene Regulation
  • Molecular Mechanisms

Background:

  • Polycomb Repressive Complex 1 (PRC1) and SWI/SNF are key chromatin regulators.
  • Their opposing roles in gene expression are vital for development and differentiation.
  • The precise mechanisms of their competition at chromatin remain unclear.

Purpose of the Study:

  • To elucidate how PRC1 and SWI/SNF compete for chromatin regulation.
  • To investigate the role of PRC1's condensate-forming properties in this antagonism.
  • To understand the interplay between PRC1 condensates and SWI/SNF binding.

Main Methods:

  • Employed single-molecule analysis in cultured cells.
  • Utilized genomic approaches to map complex interactions.
  • Assessed PRC1 condensate formation propensity and SWI/SNF binding dynamics.

Main Results:

  • PRC1's ability to form condensates is crucial for excluding SWI/SNF from chromatin.
  • PRC1 variants with higher condensate formation propensity more effectively block SWI/SNF.
  • SWI/SNF binding reduces PRC1 binding and subsequent condensate formation, independent of ATP hydrolysis.

Conclusions:

  • PRC1 condensate formation drives mutual antagonism with SWI/SNF.
  • This dynamic interplay ensures balanced regulatory activities during development.
  • Condensate properties are central to resolving competing chromatin regulatory complex functions.