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Updated: May 11, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
Published on: July 26, 2010
DNA frameworks equipped with high-affinity multivalent aptamers for the reliable and amplified detection of a
Zhimei He1, Ziyi Zhou1, Qin Fan1
1State Key Laboratory of Flexible Electronics (LoFE), Institute of Advanced Materials (IAM), Jiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology, School of Chemistry and Life Sciences, Nanjing University of Posts and Telecommunications, Nanjing 210023, China. iamjchao@njupt.edu.cn.
Abstract:
A tetrahedral DNA framework-based fluorescent probe is programmed with multivalent aptamers for the precise targeting of membrane nucleolin and an initiator strand to trigger a hybridization chain reaction. Benefiting from multivalent aptamer targeting and HCR signal amplification, this probe demonstrates minimal endocytosis, enabling the reliable and amplified detection of a membrane protein biomarker.
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