PuRBOHF-PuPRX42-like complex activates lignin biosynthesis for stone cell formation in pear fruit

Area of Science:

• Plant Biology
• Fruit Quality Research
• Biochemistry

Background:

• Lignin deposition in stone cells significantly impacts pear fruit quality.
• NADPH oxidase (RBOH) generates reactive oxygen species (ROS), regulating plant physiological processes.
• The genetic regulation of RBOH in stone cell lignin biosynthesis is not well understood.

Purpose of the Study:

• To elucidate the molecular mechanisms of RBOH-regulated lignin biosynthesis during stone cell formation in pear fruit.
• To investigate the role of PuPRX42-like and PuRBOHF in stone cell development.

Main Methods:

• Gene expression analysis of PuPRX42-like and PuRBOHF in pear fruit tissues.
• Quantification of stone cell content.
• Investigation of protein-protein interactions between PuRBOHF and PuPRX42-like.

Main Results:

• Stone cell content increased with PuPRX42-like expression.
• Stage-specific expression of PuRBOHF was higher in stone cells, correlating with increased ROS production and lignin biosynthesis.
• A PuRBOHF-PuPRX42-like complex was identified, which promotes lignin biosynthesis and stone cell formation.

Conclusions:

• PuRBOHF acts as a positive regulator in stone cell development.
• The interaction between PuRBOHF and PuPRX42-like is crucial for promoting lignin biosynthesis and stone cell formation in pear fruit.
• This study clarifies the molecular pathways governing lignin deposition in pear stone cells.