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Separation of bacterial luciferase from oxidoreductases by affinity chromatography
Analytical Biochemistry
|November 15, 1985
Abstract:
The NADH-dependent and NADPH-dependent oxidoreductase activities associated with bacterial luciferase in Vibrio (Beneckea) harveyi can simultaneously be removed from purified luciferase through a Blue Sepharose CL-6B column. The result achieved with this one-step affinity chromatography is similar to that obtained with two sequential "reverse-affinity" chromatographies.