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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.9K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.9K
Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

3.2K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
3.2K
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

858
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
858
Nucleic acids02:43

Nucleic acids

167.9K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
167.9K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.3K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.3K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

501
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
501

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Related Experiment Video

Updated: Sep 8, 2025

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
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Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

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In-cell NMR spectroscopy of nucleic acids: Basic concepts, practical aspects, and applications.

Silvie Foldynova-Trantirkova1, Jakub Harnos2, Jan Rynes1

  • 1Central European Institute of Technology, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic.

Progress in Nuclear Magnetic Resonance Spectroscopy
|September 5, 2025
PubMed
Summary

In-cell NMR spectroscopy reveals nucleic acid behavior within living cells, highlighting differences from in vitro studies. This review guides sample preparation and data interpretation for cellular structural biology.

Keywords:
Cellular structural biologyDNAHuman cellsIn-cell NMRRNAXenopus oocytes

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Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
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Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
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Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
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Area of Science:

  • Structural Biology
  • Biophysics
  • Molecular Biology

Background:

  • In-cell NMR spectroscopy provides atomic-level insights into nucleic acids (NAs) inside living cells.
  • Recent studies reveal distinct NA behavior in cellular environments versus in vitro conditions.
  • Understanding cellular states is crucial for interpreting NA dynamics.

Purpose of the Study:

  • To review the principles and practicalities of in-cell NMR data acquisition.
  • To discuss sample preparation and manipulation techniques for eukaryotic models (Xenopus laevis oocytes, human cells).
  • To guide the planning and presentation of in-cell NMR experiments on nucleic acids.

Main Methods:

  • Focuses on established eukaryotic cellular models for in-cell NMR.
  • Details sample preparation and manipulation strategies for data acquisition.
  • Covers experimental planning and data presentation guidelines.

Main Results:

  • In-cell NMR uncovers fundamental differences in NA behavior within cells compared to in vitro.
  • Data acquisition in Xenopus laevis oocytes and human cells is detailed.
  • Technical and conceptual aspects of sample handling are vital for physiological relevance.

Conclusions:

  • In-cell NMR is a powerful tool for understanding NA structure and dynamics in their native cellular context.
  • This review empowers readers to critically assess and conduct in-cell NMR studies.
  • The field of cellular structural biology is rapidly expanding, with in-cell NMR at its forefront.