Related Experiment Video
Updated: Sep 8, 2025
Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses
Published on: November 3, 2014
Scorpion venom gland transcriptomics: A systematic review
Jennifer Alexandra Solano-Godoy1, Melissa Betancourt-Osorio2, Marcela Orjuela-Rodriguez2
1Grupo Infección e Inmunidad, Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira, Colombia; Grupo de Investigaciones Herpetológicas y Toxinológicas (GIHT), Universidad del Cauca, Popayán, Colombia.
Abstract:
Scorpion venom is a complex biochemical arsenal with significant ecological and biomedical importance. Advances in transcriptomic techniques have provided valuable insights into the composition and functional diversity of venoms. This systematic review analyzes transcriptomic research conducted between 2010 and 2024, focusing on methodologies such as Expressed Sequence Tags (ESTs) and Next-Generation Sequencing (NGS). 42 studies from four databases (Scopus, PubMed, Web of Science, and ScienceDirect) were selected, showcasing their impact on detecting venom-related transcripts. The classification of venom components revealed a diverse range of bioactive molecules, including ion channel toxins, enzymes, antimicrobial peptides, and uncharacterized components with potential therapeutic applications. The findings emphasize the importance of multi-omic approaches in elucidating venom complexity and the potential applications of novel components in pharmacology and biotechnology. However, significant challenges remain, including the need for standardization and expanded research on underrepresented taxa. This review highlights the necessity for interdisciplinary efforts to bridge these gaps and fully harness the potential of scorpion venom studies.
