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Nine-year microflora study of an isolator-maintained immunodeficient child
Applied and Environmental Microbiology
|December 1, 1985
Summary
This 9-year study monitored a child's microbiome, identifying distinct permanent and transient microbial communities. Key bacterial and fungal species established long-term colonization, offering insights into human microbial ecology.
Area of Science:
- Microbiology
- Human Microbiome Research
- Environmental Microbiology
Background:
- Understanding the establishment and dynamics of the human microbiome is crucial for health.
- Longitudinal studies are essential to track microbial community development in controlled settings.
Observation:
- A male child's microbial colonization was monitored for nine years in an isolator.
- Samples from the mouth, nasal passages, feces, and body surfaces were analyzed monthly for bacteria, yeasts, and fungi.
- Three distinct microbial categories—incident, transient, and permanent—were identified.
Findings:
- Permanent residents included *Penicillium citrinum* and 17 bacterial types, notably alpha-hemolytic streptococci and *Staphylococcus epidermidis*.
- Transient microorganisms were detected but did not establish long-term residence.
- The number of colony-forming units (CFUs) and species diversity in feces were comparable to healthy adults, while other sites showed lower diversity.
Implications:
- This study provides a detailed view of microbial community development in an isolated human host.
- Findings contribute to understanding factors influencing microbial colonization and stability.
- The data can inform future research on microbiome-host interactions and the impact of environmental controls.