Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

773.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
773.3K
Next-generation Sequencing03:00

Next-generation Sequencing

97.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.9K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.6K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

601
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
601

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Forensic evaluation of 101 identity-informative SNPs in a Filipino population using massively parallel sequencing.

Forensic science international. Genetics·2026
Same author

Short tandem repeat (STR) typing of a deceased individual with an extensive blood transfusion history: A case report.

Practical laboratory medicine·2026
Same author

Insights into the mitochondrial DNA genetic diversity and affinities of village chickens from the Sulu Archipelago, Philippines.

Royal Society open science·2025
Same author

Barriers to human remains identification using forensic odontology in resource-constrained settings.

Forensic science international. Synergy·2025
Same author

Bringing the science back into forensic science in death investigations in the Philippines during the COVID-19 pandemic.

Forensic Science International. Reports·2023
Same author

Strengthening mental health initiatives and care pathways for families affected by the Philippine drug war.

The Lancet regional health. Western Pacific·2023

Related Experiment Video

Updated: Jan 18, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

22.9K

Challenges in using massively parallel sequencing technology for forensic DNA analysis in Southeast Asia.

Christian S Estrella1, Maylowen P Lumayna1, Minerva D Sagum1

  • 1DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman, Quezon City 1101 Philippines.

Forensic Science International. Synergy
|September 8, 2025
PubMed
Summary

Massively parallel sequencing (MPS) adoption in Southeast Asian forensic labs is limited due to challenges like data and infrastructure. Recommendations include strategic implementation and regional collaboration for wider use.

Keywords:
AFSNChallengesDNAForensicsMPSNGSSoutheast Asiamassively parallel sequencing

More Related Videos

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.6K
Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

6.9K

Related Experiment Videos

Last Updated: Jan 18, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

22.9K
Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.6K
Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

6.9K

Area of Science:

  • Forensic Science
  • Genetics
  • Molecular Biology

Background:

  • Massively parallel sequencing (MPS) offers higher resolution in forensic DNA analysis.
  • Adoption of MPS technology in Southeast Asian forensic laboratories remains limited.
  • Existing national DNA databases often rely on short tandem repeat (STR) markers analyzed via capillary electrophoresis (CE).

Purpose of the Study:

  • To review the current implementation of MPS in forensic DNA laboratories across Southeast Asia.
  • To identify and discuss the key challenges hindering MPS adoption in the region.
  • To propose strategies for expanding the use of MPS in forensic casework and databasing.

Main Methods:

  • An online survey was conducted with the Asian Forensic Sciences Network - DNA Workgroup (AFSN-DNAWG).
  • Nineteen responses were collected from forensic DNA laboratories in seven Southeast Asian countries.
  • Survey data was analyzed to identify current MPS users, non-users, and adoption challenges.

Main Results:

  • Eight of the surveyed laboratories are currently using MPS, while ten non-users plan adoption within five years.
  • Key challenges for MPS users include limited population data, lack of standardized nomenclature, and database incompatibility.
  • Non-users face barriers such as limited infrastructure, financial constraints, and insufficient training.

Conclusions:

  • A phased approach combining CE-based profiling for routine cases and MPS for complex cases is recommended.
  • Establishing dedicated MPS-capable forensic laboratories per country and fostering regional collaboration are crucial.
  • These strategies aim to facilitate the integration of MPS into forensic databasing and casework across Southeast Asia.