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Updated: Jan 18, 2026
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
Published on: November 20, 2017
Biological properties of valve materials using RGD and EC
Chennian Xu1,2,3, Yang Liu2, Rui Qiao4
1Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi'an, 710032, Shaanxi, China.
Background:
Rapid application of transcatheter valve replacement for valve diseases has promoted the development of biological valves. Different efforts have been made to improve the surface properties of valves. We developed a new method using arginine-glycine-aspartate (RGD) peptide and epoxy chloropropane (EC). Our goal was to evaluate and detect the surface biological characteristics of valve materials using RGD and EC treatments.
Methods:
The surfaces of the valve materials used in the GA-EC and RGD-EC groups were smooth and relatively dense, as observed using a scanning electron microscope.
Results:
More MSCs adhered to and grew on the samples in the GA-EC and RGD-EC groups than in the GA group. The apoptosis rate of MSCs was markedly decreased, whereas the expression of vimentin was elevated in the GA-EC and RGD-EC groups (P < 0.05). The adherent ability of MSCs in the RGD-EC and GA-EC groups was significantly higher than that in the GA group (P < 0.05).
Conclusions:
The new treatment method using RGD and EC improves the biological properties of the surface of the biological valve materials.
