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[Fragments of limited trypsinolysis of native alpha-actinin]
Bioorganicheskaia Khimiia
|November 1, 1985
Abstract:
Incubation of native alpha-actinin with trypsin leads to the formation of intermediate fragments that are quite resistant to further enzyme action (M 98, 85, 80, 70, 64, 55, 38, 30, 15 kDa). Analysis of these fragments made it possible to elucidate the course of trypsinolysis and to localize the fragments in the polypeptide chain of alpha-actinin subunit.