[Experimental optimization of paraffin sectioning techniques for the eyeball].

F F Huang1, M M Du1, N Gao1

  • 1Ophthalmology Medical Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory for the Prevention and Treatment of Major Blinding Eye Diseases, Chongqing Branch (Municipality Division) of National Clinical Research Centre for Ocular Diseases, Chongqing 400016, China.

Summary

Optimized eyeball paraffin sectioning uses a clinical pre-treatment and a paraformaldehyde-glutaraldehyde mixture at 4°C. This method significantly improves histological visualization of key ocular structures for better histopathological evaluation.