Effects of α-Lipoic Acid, vitamin E, and Selenium Combination on Inflammation-Independent ROS-Induced Damage in
Ayse Karatug Kacar1, Onur Ertik2,3, Nilay Dinckurt4,5
1Department of Biology, Faculty of Science, Istanbul University, Istanbul, Türkiye.
Abstract:
Diabetes is a metabolic and chronic disease affecting different tissues' metabolism. Genetic factors, lifestyles, and dietary habits can cause it. In diabetes, oxidative stress can occur in metabolic disorders, negatively affecting it. The antioxidants are essential in reducing or completely stopping the harmful effects of these adverse effects on the tissues. In the present study, we aimed to determine the combined effects of lipoic acid, vitamin E, and selenium in the kidneys of diabetic mice. For this experiment, the Balb/c mice were used and divided into five groups: citrate buffer, the solvents of the antioxidants, combined the antioxidants (α-lipoic acid, vitamin E, and selenium), streptozotocin, combined with the antioxidants and streptozotocin (A+D). At the end of 30 days of this process, the mice were sacrificed by cervical dislocation. Kidney tissues were taken for morphological, Western blotting, and biochemical analyses. The tissue was used for staining with Masson's trichrome and periodic acid-Schiff (PAS) of renal tissue sections taken for histological analysis; Western blotting such as the level of IL-10, IL-1β, TGF-β, p38, cCas3, NRF2; biochemical parameters such as the level of GSH, LPO, SOD, CAT, GR, TAS, TOS, ROS, OSI, PON, CA, LDH, AR, ADA, arginase, OH-proline, and AOPP. The histological findings showed mild damage to the kidney tissue of diabetic mice. Western blot results showed that the damage was independent of inflammation. Biochemical results revealed that administering combined antioxidants to diabetic mice protects the kidney tissue.
Insights
Combined antioxidants, including lipoic acid, vitamin E, and selenium, protect kidney tissue in diabetic mice. This study shows these antioxidants mitigate oxidative stress and kidney damage in diabetes.
Area of Science:
- Metabolic and chronic disease research
- Oxidative stress and antioxidant mechanisms
- Renal tissue analysis
Background:
- Diabetes mellitus is a chronic metabolic disorder impacting tissue metabolism.
- Oxidative stress is a key factor in diabetes complications, including kidney damage.
- Antioxidants play a crucial role in mitigating cellular damage caused by oxidative stress.
Purpose of the Study:
- To investigate the protective effects of combined antioxidants (lipoic acid, vitamin E, selenium) on kidney tissue in diabetic mice.
- To evaluate the impact of these antioxidants on histological, molecular, and biochemical markers of kidney damage.
- To assess the role of oxidative stress in diabetic nephropathy.
Main Methods:
- Balb/c mice were divided into five groups: control, antioxidant solvent, combined antioxidants, streptozotocin-induced diabetes, and diabetes with antioxidant treatment.
- Kidney tissues were analyzed using Masson's trichrome and PAS staining for histology.
- Western blotting was performed to measure inflammatory and apoptotic markers (IL-10, IL-1β, TGF-β, p38, cCas3, NRF2).
- Biochemical assays assessed oxidative stress markers (GSH, LPO, SOD, CAT, GR, TAS, TOS, ROS, OSI) and kidney function indicators.
Main Results:
- Histological analysis revealed mild kidney damage in diabetic mice.
- Western blot data indicated that the observed kidney damage was not primarily driven by inflammation.
- Biochemical analyses demonstrated that combined antioxidant administration significantly protected kidney tissue from diabetic damage, reducing oxidative stress markers.
Conclusions:
- Combined administration of lipoic acid, vitamin E, and selenium offers significant protection to kidney tissue in a mouse model of diabetes.
- Antioxidants effectively combat oxidative stress, a critical factor in diabetic nephropathy.
- The findings support the therapeutic potential of antioxidant combinations in managing diabetic kidney complications.
