Revolutionizing meropenem detection: novel OVA-PtNP nanoenzymes for clinical and environmental safety

Purpose: The objective of this study is to develop a straightforward and expeditious clinical detection method for meropenem. This study aims to introduce an innovative nanoenzyme design, thereby broadening the application of platinum nanomaterials in biological detection. It seeks to facilitate the portable detection of meropenem using commercial software. Methods: Ovalbumin platinum nanoparticle units (OVA-PtNPs) were synthesized via a redox method, utilizing ovalbumin as a stabilizer for meropenem detection. The OVA-PtNPs induced a color change in TMB substrates, resulting in the formation of oxidized TMB with a strong ultraviolet absorption peak at 652 nm. Upon the addition of meropenem, the absorbance at this wavelength decreased, thereby enabling the detection of meropenem. Results: The experimental results confirmed that OVA-PtNPs were successfully prepared with a stable structure and significant oxidase-like activity, generating substantial superoxide anions (O₂˙^-). This method could detect meropenem in the range of 3.125-500 μg mL^-1, with a detection limit of 0.75 μg mL^-1. Furthermore, the meropenem concentrations in three groups of serum samples analyzed through spiked recovery experiments showed a relative standard deviation (RSD) of less than 3%. Conclusion: This research presents novel concepts for the design of nanoenzymes and the expanded application of platinum nanomaterials in biological detection.