Repeatability of diffusion-weighted arterial spin labeling MRI for mapping blood-brain barrier water exchange rate at...

This study sought to determine the intrasession repeatability of the diffusion-weighted (DW) arterial spin labeling (ASL) sequence at different postlabel delays (PLDs).

We first performed numerical simulations to study the accuracy of the two-compartment water exchange rate (Kw) fitting model with added Gaussian noise for DW PLDs at 1500, 1800, and 2100 ms. Ten young, healthy participants then underwent a structural T₁ scan and two intrasession in vivo DW ASL scans at each PLD on a 3T MRI. The Kw, arterial transit time (ATT), and cerebral blood flow maps were linearly registered to the structural images, which were then segmented using FreeSurfer into masks with 35 bilateral gray-matter regions.

Simulation results showed that the Kw fitting model performed at an error rate less than 10% at physiological ATTs and Kw values, but that error and bias increased at a PLD of 2100 ms and at ATT ranges where the overall blood signal fraction (A₁) is low. In vivo analysis showed a significant positive correlation between intrasession measurements of regional Kw at a DW PLD of 1800 ms (β = 0.33, p < 0.001) only. Furthermore, a significant positive relationship between Kw and cerebral blood flow was seen at a DW PLD of 1500 ms (β = 0.26, p = 0.005) and DW PLD of 2100 ms (β = 0.39, p = 0.006).

Overall, DW ASL provides the strongest intrasession repeatability at a PLD of 1800 ms in young, healthy subjects, and a simulation study shows accurate Kw fits at physiologic range of ATTs and Kw values.