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Related Experiment Video

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Democratizing Organ-On-Chip Technologies With a Modular, Reusable, and Perfusion-Ready Microphysiological System.

Daniel J Minahan1, Katherine M Nelson2, Filipa Ribeiro1

  • 1Department of Biomedical Engineering, University of Delaware, Newark, DE, 19716, USA.

Advanced Healthcare Materials
|September 11, 2025
PubMed
Summary
This summary is machine-generated.

A new modular organ-on-chip platform simplifies microphysiological systems (MPS) fabrication and use. This adaptable system enhances reproducibility and accessibility for advanced in vitro model development.

Keywords:
in vitro tissue modelslow‐resourcemicrophysiological system (MPS)modular microfluidicsorgan‐on‐chip (OOC)

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Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Microfluidics

Background:

  • Organ-on-chip (OOC) and microphysiological systems (MPS) offer dynamic cell culture but face adoption barriers due to complex fabrication, material limitations (e.g., polydimethylsiloxane), and poor modularity.
  • Existing static cell culture methods lack the physiological relevance of dynamic microenvironments crucial for advanced in vitro modeling.
  • Widespread use of OOC/MPS is limited by fabrication complexity, material constraints, and lack of modularity, hindering broader scientific adoption.

Purpose of the Study:

  • To present a novel, modular microphysiological system (MPS) platform designed for ease of use, enhanced reproducibility, and broad applicability in organ-on-chip development.
  • To decouple model establishment from perfusion experiments, streamlining workflows for researchers.
  • To establish a generalizable framework for modular tissue-chip development adaptable to diverse organ systems.

Main Methods:

  • Development of a modular MPS platform using layered elastomeric inserts for dual monolayer cell culture within a reusable acrylic cassette for perfusion.
  • Validation using dual epithelial and endothelial cell co-culture under static and perfused conditions, including shear stress application.
  • Utilizing vinyl cutting for reproducible manufacturing and material testing for biocompatibility assessment.

Main Results:

  • The platform demonstrated high manufacturing fidelity and biocompatibility, supporting long-term cell culture (up to 14 days).
  • Successful co-culture of epithelial and endothelial cells was achieved, with observed shear-induced alignment of HUVECs under perfusion.
  • The modular design facilitated uniform cell seeding, imaging access, and parallelized experimentation with minimized pump usage.

Conclusions:

  • The developed modular MPS platform overcomes key limitations of current OOC technologies, offering a user-friendly, reproducible, and versatile solution.
  • This approach democratizes advanced in vitro model systems, making them accessible to labs lacking specialized microfabrication infrastructure.
  • The platform serves as a foundational framework for developing diverse organ-specific tissue chips, advancing in vitro research.