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Related Concept Videos

IR Spectrometers01:25

IR Spectrometers

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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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Infrared (IR) Spectroscopy: Overview01:09

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When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
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IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

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IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
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IR Spectrum01:19

IR Spectrum

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When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...
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Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

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The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
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Broadband Fourier-Transform Optical Photothermal Infrared Spectroscopy and Imaging.

Aleksandr Razumtcev1,2, Gwendylan A Turner1,2,3, Sergey Zayats4

  • 1Advanced Light Source Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Analytical Chemistry
|September 11, 2025
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Summary
This summary is machine-generated.

This study introduces a new optical photothermal infrared (O-PTIR) microscopy technique. It combines synchrotron infrared radiation with O-PTIR for high-resolution chemical imaging across a wide spectral range, improving on existing methods.

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Area of Science:

  • Spectroscopy
  • Microscopy
  • Chemical Imaging

Background:

  • Infrared (IR) spectroscopy maps chemical heterogeneity but is diffraction-limited.
  • Synchrotron IR sources offer high brightness and broad bandwidth.
  • Submicrometer spatial resolution is needed for advanced studies.

Purpose of the Study:

  • To extend the spectral range of photothermal infrared measurements.
  • To develop a combined synchrotron-based O-PTIR modality.
  • To achieve high spatial resolution chemical imaging across the mid-IR range.

Main Methods:

  • Incorporated a synchrotron IR source into optical photothermal IR (O-PTIR) microscopy.
  • Employed modulated IR and visible probe laser beams for detection.
  • Utilized step-scan interferometry for both optical- and fluorescence-detected modalities.

Main Results:

  • Demonstrated high spatial resolution chemical imaging spanning the mid-IR range (541-4000 cm-1).
  • Achieved improved spectral range compared to commercial laser sources.
  • Showcased improved spatial resolution compared to synchrotron microspectroscopy.

Conclusions:

  • Synchrotron-based O-PTIR enables high spatial resolution far-field chemical imaging.
  • The technique successfully differentiated cells in mouse brain tissue sections.
  • This modality overcomes IR diffraction limits for submicron chemical differentiation.