A Simple and Cost-Effective Setup to Analyze Optokinetic Response in Adult Zebrafish

The optokinetic response (OKR) is a widely used measure of visual and neurological function in zebrafish (Danio rerio). In this study, we employed a simple and cost-effective OKR assay system for adult zebrafish utilizing a mobile phone and a stereomicroscope. The setup was validated by comparing control fish with those exposed to light-induced retinal degeneration (LIRD), demonstrating significantly impaired OKR responses. This custom-made setup offers valuable applications in visual neuroscience, disease modeling, and drug discovery.