Related Experiment Video
Updated: Jan 18, 2026
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
Published on: May 25, 2018
Protocol to perform assay for chromatin-bound condensates by exploratory sequencing in mouse embryonic stem cells
Zeran Jia1, Jiawei Yu1, Yinqing Li1
1IDG/McGoven Institute for Brain Research, State Key Laboratory of Molecular Oncology, MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China.
None:
Chromatin-related protein condensates are integral to nuclear functions including transcriptional regulation and chromatin structure. Here, we present a protocol for detecting the occupancy of chromatin-associated condensates using the assay for chromatin-bound condensates by exploratory sequencing (ACC-seq) in mouse embryonic stem cells (mESCs). The workflow encompasses cell preparation, condensate modulation, library construction, and comprehensive sequencing analysis, enabling unbiased, genome-wide mapping of potential condensate occupancy across diverse chromatin states. For complete details on the use and execution of this protocol, please refer to He et al.1.
More Related Videos
08:53Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
Published on: May 2, 2025
08:40High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease ChIP-Exo
Published on: August 14, 2020