Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of Menopausal Hormone Therapy on Uterine Fibroids in Women During Menopausal Transition.

International journal of women's health·2026

Same author

Timed melatonin administration increases territorial but not non-territorial aggression in female Siberian hamsters.

Hormones and behavior·2026

Same author

Black phosphorus nanosheet-enabled ROS-adaptive core-shell microneedles orchestrate immunoredox remodeling and neurotrophic regeneration for facial nerve repair.

Journal of nanobiotechnology·2026

Same author

Host genetic regulation of rumen 6-hydroxymelatonin reduces methane emissions in dairy cattle.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Phaco tip-chopper flip technique for epinucleus removal in cataract surgery.

BMC ophthalmology·2026

Same author

PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.

Cell death & disease·2026

Search research articles

Related Experiment Video

Updated: Jul 16, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

CRISPR/Cas12-Driven Exponential Amplification Combined with a Lateral Flow Biosensor Enabling Rapid and Highly

Mengqi Huang¹, Kunte Shang¹, Luyi Ying¹

¹Department of Ophthalmology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.

|September 12, 2025

Summary

A new CRISPR-Cas12 biosensor (CADEX-LF) offers rapid, highly sensitive DNA detection. This point-of-care technology shows promise for diagnosing diseases like HSV-1, even in resource-limited settings.

Keywords:

CRISPR/Cas12 HSV-1 detection exponential amplification lateral flow biosensor point-of-care diagnosis

More Related Videos

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

Published on: July 18, 2025

Related Experiment Videos

Last Updated: Jul 16, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

Published on: July 18, 2025

Area of Science:

Biotechnology
Molecular Diagnostics
Biosensor Technology

Background:

Accurate DNA detection is crucial for research and clinical diagnostics.
CRISPR technology enhances DNA testing speed, portability, and accuracy.
Existing methods may lack sensitivity or require complex equipment.

Purpose of the Study:

To develop a rapid and highly sensitive DNA detection biosensor.
To leverage Cas12 activity and exponential amplification for enhanced detection.
To create a point-of-care (POC) compatible diagnostic tool.

Main Methods:

Developed a Cas12-driven exponential amplification-based lateral flow biosensor (CADEX-LF).
Utilized Cas12's target-loading-dependent *trans*-cleavage activity.
Employed nicking endonuclease-mediated exponential amplification for signal enhancement.

Main Results:

Achieved a detection sensitivity of 2 × 10^-15 M within 45 minutes.
Demonstrated outstanding specificity with double-base resolution.
Successfully identified herpes simplex virus 1 (HSV-1) DNA in clinical samples.

Conclusions:

CADEX-LF provides rapid, sensitive, and specific DNA detection.
The biosensor is suitable for point-of-care applications, requiring no complex equipment.
CADEX-LF shows significant potential for clinical diagnosis, particularly in resource-limited environments.