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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Updated: Jan 18, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
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Engineering Conditional Transgene Expression in Nicotiana benthamiana.

Elena Garcia-Perez1, Marta Vazquez-Vilar1, Diego Orzaez1

  • 1Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Valencia, Spain.

Plant Biotechnology Journal
|September 12, 2025
PubMed
Summary
This summary is machine-generated.

Synthetic biology advances N. benthamiana (Nicotiana benthamiana) as a plant biofactory. Gene circuits overcome challenges like low yields and transgene silencing for scalable molecular farming and biosensing.

Keywords:
Nicotiana benthamianametabolic engineeringmolecular farmingplant synthetic biologyrecombinasessynthetic gene circuitssynthetic promoters

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Area of Science:

  • Plant biotechnology
  • Synthetic biology
  • Molecular farming

Background:

  • Nicotiana benthamiana is a key plant biofactory for producing proteins and metabolites.
  • Challenges include transgene silencing, low yields, and metabolic toxicity, limiting scalability.
  • Synthetic gene circuits offer precise control over transgene expression and metabolic pathways.

Purpose of the Study:

  • To review advancements in synthetic biology for enhancing Nicotiana benthamiana as a biofactory.
  • To highlight solutions for overcoming limitations in plant-based bioproduction.
  • To explore future strategies for optimizing N. benthamiana for molecular farming and biosensing.

Main Methods:

  • Development of novel sensor systems for chemical and electromagnetic signals.
  • Integration of synthetic promoters with programmable transcription factors.
  • Utilizing virus-derived replicons for transcriptional amplification.
  • Employing recombinase-based processors for conditional gene expression and cellular memory.
  • Implementing innovative platforms like plant cell packs and bioluminescence systems for rapid prototyping and high-throughput screening.

Main Results:

  • Synthetic gene circuits enable precise control, dynamic signal processing, and metabolic pathway optimization.
  • Advancements facilitate rapid prototyping and optimization of gene circuits.
  • Novel systems enhance gene expression control and cellular memory.
  • Progress in addressing challenges like transgene silencing and low yields.

Conclusions:

  • Synthetic biology offers transformative solutions for N. benthamiana-based bioproduction.
  • Future strategies focus on stable genomic integration and accelerated transgenic line generation.
  • Optimized N. benthamiana can unlock potential for scalable molecular farming, biosensing, and bioproduction.