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Related Experiment Video

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Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials
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Microgel Aspect Ratio Influences Injectable Granular Hydrogel Scaffold Pore Structure and Cellular Invasion for

Gabriel J Rodriguez-Rivera1, Siddharth Sharma2, Chima V Maduka1

  • 1BioFrontiers Institute, University of Colorado Boulder, Boulder, CO, 80309, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 12, 2025
PubMed
Summary

Using rod-like hyaluronic acid microgels, researchers created highly porous granular hydrogels. These advanced scaffolds enhance cellular infiltration and show promise for treating myocardial infarction, improving cardiac function.

Keywords:
cellular invasiongranular hydrogelsporositytissue repairvoid fraction

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Granular hydrogels are promising scaffolds for biomedical uses due to injectability and porous structure.
  • Previous studies on spherical microgels offered limited control over porosity.
  • Microgels with higher aspect ratios present an opportunity for enhanced porosity.

Purpose of the Study:

  • To investigate the effect of hyaluronic acid microgel aspect ratio on granular hydrogel porosity and cellular interactions.
  • To compare the performance of rod-like microgels versus spherical microgels in granular hydrogel formation.
  • To evaluate the therapeutic potential of high-aspect-ratio microgels in a myocardial infarction model.

Main Methods:

  • Fabrication of hyaluronic acid microgels with varying aspect ratios (3-5).
  • Characterization of granular hydrogel porosity using simulations and experimental methods.
  • Assessment of cellular infiltration using endothelial cell spheroid migration assays.
  • In vivo evaluation in a rat model of myocardial infarction.

Main Results:

  • Rod-like microgels significantly increased void volume fraction and pore size compared to spherical microgels.
  • Enhanced cellular invasion was observed in granular hydrogels made from rod-like microgels.
  • Injectable granular hydrogels with high-aspect-ratio microgels reduced infarct area and improved cardiac function in rats.

Conclusions:

  • Microgel shape is a critical factor in designing injectable granular hydrogels with controlled porosity.
  • Rod-like microgels offer superior properties for cellular infiltration and tissue regeneration.
  • This study provides insights for developing advanced hydrogel scaffolds for therapeutic applications.