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Non-Invasive Human-Free Diagnosis Methods for Assessing Pig Welfare at Abattoirs: A Systematic Review
Maria Francisca Ferreira1, Márcia Nunes2, Madalena Vieira-Pinto1,3
1CECAV-Veterinary and Animal Research Centre, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal.
Animals : an Open Access Journal From MDPI
|September 13, 2025
Summary
Non-invasive methods for assessing pig welfare and health at abattoirs are being explored. While challenges remain, blood analysis and automated imaging show promise for practical integration into slaughter lines.
Area of Science:
- Animal Science
- Veterinary Medicine
- Food Safety
Background:
- Traditional pig welfare and health assessments at abattoirs rely on subjective, time-consuming human observation.
- High-throughput facilities require scalable, objective, and non-invasive methods for efficient evaluation.
- Ensuring animal well-being and food safety necessitates improved diagnostic tools in commercial settings.
Purpose of the Study:
- To systematically review and evaluate non-invasive, human-free diagnostic methods for pig welfare and health assessment in abattoirs.
- To identify and categorize emerging technologies applicable to commercial slaughter operations.
- To assess the implementation potential and practical challenges of these novel methods.
Main Methods:
- Systematic literature review adhering to PRISMA guidelines.
- Inclusion of 102 articles evaluating diagnostic methods for pig welfare and health.
- Classification of identified methods into biological sample analysis, imaging/computer vision, and sensor-based systems.
Main Results:
- Thirteen distinct non-invasive methods were identified across three categories.
- Blood analysis (glucose, lactate), convolutional neural networks (lesion detection), and automated camera systems show the most promise.
- No single method currently offers both high implementation and practicality, with challenges in automation and standardization.
Conclusions:
- Non-invasive diagnostic methods offer significant potential for improving pig welfare and health assessment in abattoirs.
- Continued research and validation are crucial for overcoming technical hurdles and enabling routine adoption.
- Automated, human-free technologies are key to enhancing objectivity and efficiency in abattoir practices.

