Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

88
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
88

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimizing Lysis and Extraction Workflows for Enrichment-Free qPCR Detection of <i>Salmonella enterica</i> in Poultry Matrices.

Pathogens (Basel, Switzerland)·2026
Same author

A Review of the Diagnostic Approaches for the Detection of Antimicrobial Resistance, Including the Role of Biosensors in Detecting Carbapenem Resistance Genes.

Genes·2025
Same author

Genomic nano-biosensor for rapid detection of the carbapenem-resistant gene <i>bla</i> <sub>NDM-1</sub> in carbapenemase-producing bacteria.

Nanoscale advances·2025
Same author

Parallel Detection of the Unamplified Carbapenem Resistance Genes <i>bla</i><sub>NDM-1</sub> and <i>bla</i><sub>OXA-1</sub> Using a Plasmonic Nano-Biosensor with a Field-Portable DNA Extraction Method.

Biosensors·2025
Same author

Detection of Unamplified <i>E. coli</i> O157 DNA Extracted from Large Food Samples Using a Gold Nanoparticle Colorimetric Biosensor.

Biosensors·2022

Related Experiment Video

Updated: May 2, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.6K

Integrated Sample to Detection of Carbapenem-Resistant Bacteria Extracted from Water Samples Using a Portable Gold

Kaily Kao1,2, Evangelyn C Alocilja1,2

  • 1Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.

Sensors (Basel, Switzerland)
|September 13, 2025
PubMed
Summary

A novel plasmonic biosensor with a smartphone app rapidly detects carbapenemase resistance genes in various water samples. This portable device offers real-time monitoring for effective antimicrobial resistance surveillance.

Keywords:
antimicrobial resistancebiosensorcarbapenem resistancediagnosticsgenesoptical biosensingpathogens

More Related Videos

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
09:04

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

8.1K
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

14.0K

Related Experiment Videos

Last Updated: May 2, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.6K
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
09:04

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

8.1K
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

14.0K

Area of Science:

  • Biotechnology
  • Biosensing
  • Antimicrobial Resistance

Background:

  • Antimicrobial resistance (AMR) poses a global health crisis, exacerbated by antibiotic overuse and a lack of rapid detection tools.
  • Carbapenem resistance, in particular, is a growing concern, necessitating swift surveillance methods.
  • Current methods for detecting resistance genes are often slow and require laboratory infrastructure.

Purpose of the Study:

  • To develop and evaluate a rapid, portable biosensor for detecting critical carbapenemase resistance genes.
  • To assess the biosensor's efficacy in diverse sample matrices for point-of-care (POC) applications.
  • To integrate a smartphone application for data analysis and field usability.

Main Methods:

  • A gold nanoparticle-based plasmonic biosensor was designed to detect three carbapenemase resistance genes (blaKPC-3, blaNDM-1, blaOXA-1).
  • DNA extraction was simplified using a boiling method, coupled with a portable thermal cycler.
  • Magnetic nanoparticles were employed for bacterial concentration, and optical signals were analyzed via spectrophotometry and a smartphone app (RGB values).

Main Results:

  • The biosensor successfully detected the target carbapenemase resistance genes in sterile, river, and turkey rinse water samples.
  • The limit of detection was as low as 2.5 ng/L (approximately 10^3 CFU/mL), demonstrating high sensitivity.
  • The smartphone app provided accurate quantification of color changes, facilitating data analysis.

Conclusions:

  • The developed biosensor system offers a rapid, cost-effective, and portable solution for detecting carbapenem resistance genes.
  • Its potential for point-of-care use in resource-limited settings can significantly enhance AMR surveillance and response.
  • Real-time monitoring and on-site testing capabilities enable immediate decision-making in critical situations.