Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention01:05

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

200
Body:Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
200
Drug Delivery: Overview01:16

Drug Delivery: Overview

730
The selection of a drug's delivery route depends upon its physicochemical properties, including lipid or water solubility and ionization, as well as the therapeutic requirement, such as immediate or sustained effect. These routes can be divided into three primary categories: enteral, parenteral, and topical.
Enteral delivery involves administering drugs directly through swallowing, sublingual placement, or buccal application. Orally administered drugs predominantly navigate the...
730
Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

188
Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...
188

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advanced Functional Wound Dressings in Precision Surgery: Immunometabolic Reprogramming, Bioadaptive Biomaterials, and Intelligent Regenerative Interfaces.

International journal of molecular sciences·2026
Same author

Antidiabetic Potential of <i>Aronia melanocarpa-</i>β-Glucan System: From Extraction Optimization Through In Silico Understanding of Activity to Stabilization of Anthocyanins.

Molecules (Basel, Switzerland)·2026
Same author

Toward a Therapy for Autism Spectrum Disorder: The Formyl Peptide Receptor 2 Agonist MR-39 Supports Synaptic Health in the BTBR Mouse and Features a Favorable Safety Profile.

ACS pharmacology & translational science·2026
Same author

From Biomaterials to Biological State Engineering: Reframing Advanced Wound Dressings as Adaptive Therapeutic Interfaces in Translational Medicine.

Cells·2026
Same author

Extemporaneous Cyclodextrin-Based Oral Solution of Ursodeoxycholic Acid Using a Ready-to-Use Vehicle.

Pharmaceutics·2026
Same author

Thymoquinone-Loaded Electrospun Fibrous Mats as Advanced Wound Dressing Materials.

Pharmaceutics·2026

Related Experiment Video

Updated: Jan 18, 2026

Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity
11:06

Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

Published on: March 1, 2016

10.8K

Effective and Stable Senomorphic Apigenin Delivery System Obtained by Supercritical Carbon Dioxide Processing.

Anna Stasiłowicz-Krzemień1, Natalia Rosiak1, Giuseppe Francesco Racaniello2

  • 1Department of Pharmacognosy and Biomaterials, Poznan University of Medical Sciences, Rokietnicka 3 Str., 60-806 Poznan, Poland.

International Journal of Molecular Sciences
|September 13, 2025
PubMed
Summary

This study developed a stable amorphous dispersion of apigenin (AP) using supercritical carbon dioxide and Soluplus, significantly enhancing its solubility and bioavailability. The improved formulation demonstrated superior antioxidant and neuroprotective effects, overcoming AP

Keywords:
amorphizationapigeninneuroprotectionsenomorphicsolubilitysupercritical conditions

More Related Videos

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery
09:44

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Published on: September 26, 2025

444
Preparation of Biopolymer Aerogels Using Green Solvents
08:13

Preparation of Biopolymer Aerogels Using Green Solvents

Published on: July 4, 2016

18.2K

Related Experiment Videos

Last Updated: Jan 18, 2026

Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity
11:06

Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

Published on: March 1, 2016

10.8K
Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery
09:44

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Published on: September 26, 2025

444
Preparation of Biopolymer Aerogels Using Green Solvents
08:13

Preparation of Biopolymer Aerogels Using Green Solvents

Published on: July 4, 2016

18.2K

Area of Science:

  • Pharmacology and Pharmaceutical Sciences
  • Materials Science
  • Biochemistry

Background:

  • Apigenin (AP), a natural flavonoid, possesses senomorphic and neuroprotective properties.
  • Poor aqueous solubility of AP (<1 μg/mL) severely limits its bioavailability and therapeutic applications.
  • Developing strategies to enhance AP solubility is crucial for its effective utilization.

Purpose of the Study:

  • To create a stable amorphous dispersion of apigenin (AP) using supercritical carbon dioxide (scCO2) processing.
  • To evaluate the enhanced solubility, bioavailability, and biological activities of the AP amorphous dispersion.
  • To confirm the stability and characterize the intermolecular interactions within the AP dispersion.

Main Methods:

  • Screening of AP solubilization with Soluplus (SOL) under scCO2 conditions.
  • Optimization of scCO2 processing parameters (temperature, pressure) for maximum AP solubility.
  • Characterization using X-ray powder diffraction (XRPD), PAMPA model, ATR-FT-IR, and chromatographic analysis.
  • Assessment of antioxidant (DPPH, ABTS, CUPRAC) and anticholinesterase (AChE, BChE) activities.

Main Results:

  • Soluplus significantly improved AP dissolution, reaching 8050.2 ± 35.1 μg/mL under optimized scCO2 conditions.
  • XRPD confirmed amorphization, leading to enhanced AP dissolution across acidic and neutral pH.
  • Improved membrane penetration (gastrointestinal and blood-brain barriers) and 12-month stability of the amorphous dispersion.
  • Enhanced antioxidant and neuroprotective activities (anticholinesterase inhibition) correlated with improved solubility.

Conclusions:

  • A stable amorphous AP dispersion was successfully prepared using scCO2 and Soluplus, exhibiting excellent solubility and stability.
  • The developed formulation demonstrates enhanced passive diffusion across biological membranes.
  • The improved AP dispersion offers greater potential for effective antioxidant and neuroprotective therapies.