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Updated: Jan 6, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
Published on: September 28, 2017
1H, 13C and 15N backbone resonance assignment of the RNA binding motif 39 (RBM39) tandem RNA recognition motifs
Florian Malard1,2, Léa Bouton3,4, Sébastien Campagne5,6
1Univ. Bordeaux, CNRS, INSERM, ARNA, UMR 5320, U1212, Bordeaux, 33000, France. florian.malard@gmail.com.
Abstract:
The N-terminal tandem RNA Recognition Motifs (RRM1-2) of the splicing factor RNA Binding Motif 39 (RBM39) from Homo sapiens was expressed in E. coli with 13C and 15N labeling. A nearly complete assignment of backbone 1H, 15N, and 13C resonances was obtained, along with a secondary structure prediction based on the assigned chemical shifts. The predicted secondary structures of the RBM39 tandem RRM1-2 closely matched the published solution structures of the individual RBM39 RRM1 and RRM2, and also aligned well with the AlphaFold2 model of RBM39.
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