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PCR

PCR

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: Jan 17, 2026

Rapid PCR Thermocycling using Microscale Thermal Convection

Rapid PCR Thermocycling using Microscale Thermal Convection

Published on: March 5, 2011

The thermal cycling methods for rapid PCR.

Ruihua Ding¹, Jiali Zhang², Chang Chen^1,2,3

¹Shanghai Industrial μTechnology Research Institute (SITRI), Shanghai, China.

Critical Reviews in Biotechnology

|September 15, 2025

Summary

This summary is machine-generated.

Rapid Polymerase Chain Reaction (PCR) methods accelerate DNA amplification by enhancing heating/cooling or enabling faster temperature zone transitions. These advancements aim to overcome the time limitations of traditional PCR for quicker nucleic acid detection.

Keywords:

PCR POCT microfluidics nucleic acids detection rapid heating thermal cycling

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Last Updated: Jan 17, 2026

Rapid PCR Thermocycling using Microscale Thermal Convection

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Published on: March 5, 2011

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Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

Area of Science:

Molecular Biology
Biotechnology
Biophysics

Background:

Polymerase Chain Reaction (PCR) is essential for DNA amplification but is time-consuming due to thermal cycling.
The lengthy duration of conventional PCR limits its application in time-sensitive scenarios.

Purpose of the Study:

To review and categorize methods for reducing PCR thermal cycling time.
To analyze the advantages, challenges, and commercialization of rapid PCR technologies.

Main Methods:

Categorization of rapid PCR methods into two main approaches: increasing heating/cooling power and rapid movement between temperature zones.
Analysis of specific techniques including contact/non-contact heating, microfluidics, and convective PCR.

Main Results:

Methods enhancing local heating/cooling power include resistive heating, Peltier pumps, air-blow, and plasmonics.
Methods enabling rapid temperature zone transitions involve microfluidic chips, continuous flow, and convective PCR.

Conclusions:

Rapid PCR technologies offer significant time reduction compared to conventional methods.
Future directions focus on improving sensitivity, portability, and cost-effectiveness for commercial rapid PCR systems.