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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Complete genome sequence of Pseudomonas fulva strain MTT5 isolated from the maize (Zea mays) phyllosphere
Bashir A Akhoon1, Katie Christensen1, Hanna Lefevers1
1Department of Horticulture, Martin-Gatton College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky, USA.
Abstract:
Pseudomonas fulva MTT5, isolated from maize leaf surfaces, exhibits broad-spectrum antimicrobial activity. We present the complete genome sequence of P. fulva MTT5. The 4.9 Mb genome contains 4,488 predicted genes and a GC content of 61.5%. Identified biosynthetic gene clusters highlight the strain's potential as a source of novel antibiotics.
Insights
Pseudomonas fulva MTT5, a maize-associated bacterium, shows broad antimicrobial action. Its complete genome sequence reveals gene clusters suggesting potential for new antibiotic discovery.
Area of Science:
- Microbiology
- Genomics
- Biotechnology
Background:
- The maize leaf surface harbors diverse microbial communities.
- Pseudomonas fulva MTT5, isolated from maize, demonstrates significant antimicrobial properties.
- Understanding the genetic basis of antimicrobial production is crucial for antibiotic development.
Purpose of the Study:
- To present the complete genome sequence of Pseudomonas fulva MTT5.
- To identify potential antibiotic-producing gene clusters within the P. fulva MTT5 genome.
- To explore the potential of P. fulva MTT5 as a source for novel antimicrobial compounds.
Main Methods:
- Whole-genome sequencing of Pseudomonas fulva MTT5.
- Bioinformatic analysis for gene prediction and annotation.
- Identification and characterization of biosynthetic gene clusters.
Main Results:
- The complete genome of P. fulva MTT5 is 4.9 Mb in size.
- The genome contains 4,488 predicted genes with a GC content of 61.5%.
- Several biosynthetic gene clusters with potential antimicrobial functions were identified.
Conclusions:
- The genome sequence provides a foundation for understanding P. fulva MTT5's antimicrobial capabilities.
- P. fulva MTT5 is a promising candidate for the discovery of novel antibiotics.
- Further research into the identified gene clusters could yield new therapeutic agents.
