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Identification of a Major Rice Blast Quantitative Trait Locus Containing Pita/Pi39(t)/Ptr in U.S. Black Hull Awn
Aron Osakina1,2, David Goad1, Melissa H Jia2
1Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, U.S.A.
Phytopathology
|September 15, 2025
Summary
Weedy rice possesses genetic resistance to rice blast disease, caused by Magnaporthe oryzae. A major quantitative trait locus (QTL) on chromosome 12, the Pi-ta/Pi-39(t)/Ptr locus, was identified as key to this resistance.
Area of Science:
- Plant Pathology
- Genetics
- Agronomy
Background:
- Rice blast, caused by Magnaporthe oryzae, is a major threat to global food security.
- Weedy rice has evolved natural resistance mechanisms against M. oryzae.
- Weedy rice can be a valuable resource for developing resistant cultivated rice varieties.
Purpose of the Study:
- To identify genetic loci conferring blast resistance in weedy rice.
- To analyze the inheritance of blast resistance in a weedy rice x cultivated rice population.
- To explore the potential of weedy rice for marker-assisted selection in breeding programs.
Main Methods:
- Phenotypic assessment of 183 recombinant inbred lines (RILs) against four common US blast races.
- Genotyping-by-sequencing (GBS) to generate single-nucleotide polymorphisms (SNPs).
- Quantitative trait locus (QTL) mapping using r/qtl software.
Main Results:
- Parental lines exhibited resistance to all tested blast races; RILs showed varied resistance levels.
- A linkage map was constructed using 1,498 SNPs.
- A single major QTL for blast resistance was mapped to the Pi-ta/Pi-39(t)/Ptr locus on chromosome 12.
Conclusions:
- The Pi-ta/Pi-39(t)/Ptr locus is a critical factor for blast resistance in weedy rice.
- Understanding this locus provides insights into weedy rice evolution and adaptation.
- This finding can facilitate the development of blast-resistant rice varieties via marker-assisted selection.
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