Related Experiment Video

Updated: Jan 17, 2026

Isolated Pancreatic Islet Treatment and Apoptosis Measurement
09:36

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

Published on: May 2, 2025

837

Deficiency of SCAMP5 Triggers Pancreatic β-Cell Secretory Dysfunction and Apoptosis

Yingqi Zhang1,2, Donglan Tang1, Chenxi Yang1

  • 1Shenzhen University Diabetes Institute, Shenzhen Key Laboratory of Metabolism and Cardiovascular Homeostasis, Shenzhen University Medical School, Shenzhen, 518060, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 15, 2025
PubMed

Insights

Secretory carrier membrane protein 5 (SCAMP5) is crucial for pancreatic beta-cell function in type 2 diabetes. Reduced SCAMP5 impairs insulin secretion and promotes beta-cell apoptosis, revealing a new therapeutic target.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Late-stage type 2 diabetes involves beta-cell dysfunction and apoptosis.
  • Mechanisms driving beta-cell failure remain incompletely understood.
  • The role of secretory carrier membrane protein 5 (SCAMP5) in beta-cells is unclear.

Purpose of the Study:

  • To investigate the function of SCAMP5 in pancreatic beta-cells.
  • To determine the role of SCAMP5 in diabetic beta-cell failure.
  • To elucidate the molecular mechanisms underlying SCAMP5's function in beta-cells.

Main Methods:

  • Assessed SCAMP5 expression in diabetic conditions.
  • Investigated the impact of SCAMP5 deficiency on insulin secretion and apoptosis.
  • Examined SCAMP5's interaction with voltage-dependent anion channel 1 (VDAC1).
  • Analyzed the epigenetic regulation of SCAMP5 by carbohydrate-responsive element-binding protein (ChREBP).

Main Results:

  • SCAMP5 expression is reduced in diabetic beta-cells.
  • SCAMP5 deficiency impairs insulin secretion via reduced CaV1.2 expression.
  • SCAMP5 prevents beta-cell apoptosis by inhibiting VDAC1-mediated cytochrome c release.
  • Hyperglycemia-induced ChREBP epigenetically represses SCAMP5 expression.

Conclusions:

  • SCAMP5 plays a vital role in maintaining beta-cell function and survival.
  • The ChREBP-SCAMP5 axis is a key pathway in diabetic beta-cell failure.
  • Targeting SCAMP5 may offer a novel therapeutic strategy for type 2 diabetes.
Keywords:
ChREBPSCAMP5VDAC1insulin secretionβ‐cell apoptosis

More Related Videos

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro09:33

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Published on: June 2, 2018

9.5K
Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes12:12

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

Published on: June 16, 2011

14.0K

Related Concept Videos

Insulin Secretory Vesicles01:05

Insulin Secretory Vesicles

Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
6.4K
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
2.2K
Chronic Pancreatitis I: Introduction01:24

Chronic Pancreatitis I: Introduction

The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
670
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
Caspases01:24

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
13.7K
Cells and Secretions of the Pancreas01:16

Cells and Secretions of the Pancreas

The pancreas, a vital organ within the abdominal cavity, plays dual roles in the digestive and endocrine systems, collaborating with exocrine and endocrine cells to maintain optimal digestion and blood sugar levels.
Exocrine function is carried out by acinar cells, organized into clusters known as acini. These cells contribute to digestion by releasing substantial quantities of enzyme-rich, alkaline digestive juices.
Concurrently, the dispersed clusters of endocrine cells throughout the...
4.4K