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Related Concept Videos

Arboviral Encephalitis01:25

Arboviral Encephalitis

Arboviral encephalitis refers to brain inflammation caused by arthropod-borne viruses, particularly those transmitted through mosquito vectors. Among these, West Nile virus (WNV), a member of the Flaviviridae family, is a significant public health concern. WNV is an enveloped, positive-sense, single-stranded RNA virus. Human infection typically begins when an infected mosquito introduces the virus into the dermis during feeding. The primary transmission cycle involves birds as amplifying hosts...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...

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Related Experiment Video

Updated: Jul 3, 2026

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
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Japanese Encephalitis Virus Genotype 5 Infectious Clone and Reporter System for Antiviral Evaluation.

Jae-Yeon Park1, Wataru Kamitani2, Hyun-Jin Shin1

  • 1College of Veterinary Medicine, Chungnam National University, Daejeon, South Korea.

Journal of Medical Virology
|September 16, 2025
PubMed
Summary

Researchers developed a new tool to study Japanese encephalitis virus genotype 5 (JEV5). This platform aids in developing JEV5-specific vaccines and antiviral therapies, addressing concerns about current vaccine effectiveness.

Keywords:
Japanese encephalitis virusReporter virusantiviral screening assaygenotype 5infectious clone

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Area of Science:

  • Virology
  • Molecular Biology
  • Vaccinology

Background:

  • Japanese encephalitis virus genotype 5 (JEV5) emergence raises concerns about vaccine efficacy.
  • A need exists for tools to study JEV5 and develop targeted interventions.

Purpose of the Study:

  • To construct a full-length infectious cDNA clone of JEV5.
  • To generate JEV5 reporter viruses for replication tracking and antiviral screening.
  • To establish a reverse genetics platform for JEV5 research.

Main Methods:

  • Utilized a bacterial artificial chromosome (BAC) system for JEV5 cDNA cloning.
  • Recovered replication-competent JEV5 from the cDNA clone.
  • Introduced nanoluciferase and enhanced green fluorescent protein reporter genes.
  • Performed antiviral screening using reporter viruses and classical plaque assays.

Main Results:

  • Successfully constructed a full-length infectious JEV5 cDNA clone.
  • Generated JEV5 reporter viruses enabling real-time replication tracking.
  • Demonstrated the utility of reporter viruses in antiviral compound screening.
  • Validated antiviral efficacy of NITD008, consistent with plaque assays.

Conclusions:

  • A versatile reverse genetics platform for JEV5 has been established.
  • This platform facilitates genotype-specific vaccine and antiviral research for JEV5.
  • The findings support the development of novel JEV5 countermeasures.