Enhancing thai native chicken semen quality and fertility through kaempferia parviflora supplementation during cold...

Kaempferia parviflora (KP) extract was evaluated as a natural antioxidant supplement in rooster semen extender during 72 h of storage at 5°C. In Experiment 1, six KP concentrations (0.5-3.0 mg/mL) were tested in a split-plot CRD to determine effects on motility, viability, plasma membrane integrity, and malondialdehyde (MDA). KP at 1.5 mg/mL consistently preserved semen quality across 24-72 h and lowered MDA relative to the control (p < 0.05), whereas ≥2.5 mg/mL showed diminishing or adverse effects. In Experiment 2, fertility and hatchability were assessed after artificial insemination using semen stored 0-72 h with or without KP. The 1.5 mg/mL group yielded higher fertility and hatchability than the control at 24-72 h (p < 0.05). These results identify 1.5 mg/mL KP as an optimal, dose-dependent additive that mitigates oxidative damage and extends functional longevity of cooled semen.