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Encapsulation and Permeability Characteristics of Plasma Polymerized Hollow Particles

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Development of Cell-Derived Plasma Membrane Vesicles as a Nanoparticle Encapsulation and Delivery System.

Mahsa Kheradmandi¹, Amir M Farnoud^1,2, Monica M Burdick^1,2,3

¹Department of Chemical and Biomolecular Engineering, Russ College of Engineering and Technology, Ohio University, Athens, OH 45701 USA.

Cellular and Molecular Bioengineering

|September 18, 2025

Summary

This study introduces a novel method using cell-derived giant plasma membrane vesicles (GPMVs) to encapsulate nanoparticles, improving their delivery to cells with reduced toxicity. This biomimetic approach enhances nanoparticle delivery and minimizes side effects for potential therapeutic applications.

Keywords:

Cell plasma membrane Drug delivery Giant plasma membrane vesicles Nanoparticles

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Area of Science:

Biomaterials Science
Nanotechnology
Cell Biology

Background:

Developing non-invasive nanoparticle delivery platforms with biomimetic properties is crucial for reducing toxicity and enhancing targeting.
Existing biomimetic nanoparticle strategies face technical challenges, necessitating novel approaches.

Purpose of the Study:

To develop a novel method for encapsulating engineered nanoparticles within cell-derived giant plasma membrane vesicles (GPMVs).
To utilize these core-shell nanoparticle-GPMV structures for improved cargo delivery to cells.

Main Methods:

Giant plasma membrane vesicles (GPMVs) were generated from A549 cells via chemical induction.
Silica nanoparticles were loaded into parent cells before vesiculation.
GPMVs with encapsulated nanoparticles were evaluated for stability, membrane composition, and cellular uptake.

Main Results:

Cell-derived GPMVs stably retained encapsulated silica nanoparticles for over 48 hours.
GPMV membrane composition closely mirrored the parental cell plasma membrane.
GPMVs demonstrated efficient endocytosis and enhanced cellular uptake of nanoparticles compared to free nanoparticles.
GPMV-mediated delivery reduced nanoparticle cytotoxicity.

Conclusions:

A novel technique for loading nanoparticles into GPMVs during vesiculation was established.
GPMVs effectively encapsulate diverse nanoparticles and improve their delivery to cells with reduced toxicity.
This cell membrane-based approach offers a promising strategy for advanced nanoparticle/drug delivery systems.