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Updated: Jan 17, 2026

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers
Published on: November 8, 2024
Adiponectin inhibits chemokine-induced cell migration via direct interaction with platelet factor 4
Mohamed Elfeky1,2, Shima Goto1, Ali El-Far3
1Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan.
Abstract:
Despite adiponectin's recognized anti-inflammatory properties, its impact on cardiovascular homeostasis involves poorly defined mechanisms. We investigated the effect of adiponectin on chemokine-induced cell migration and their potential intermolecular interactions. Our findings revealed that cell migration induced by recombinant PF4, MCP-1, or RANTES in HL-60 cells was significantly inhibited by pre-treating cells with adiponectin. Surface plasmon resonance analysis and molecular docking analysis indicated that only PF4 binds to adiponectin with a higher affinity of adiponectin to the PF4 binding site respectively. These results suggest that adiponectin's atheroprotective functions may be mediated by its ability to reduce PF4-induced monocyte migration through direct interaction.
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