Screening and Validation of Genes Associated With Lysosomal-Dependent Cell Death in Colorectal Cancer

Area of Science:

• Oncology
• Cell Biology
• Genetics

Background:

• Colorectal cancer (CRC) remains a significant health challenge.
• Lysosome-dependent cell death (LDCD) pathways are implicated in cancer progression.
• Identifying novel therapeutic targets and prognostic indicators in CRC is crucial.

Purpose of the Study:

• To investigate the correlation between CRC and LDCD.
• To identify potential therapeutic targets and prognostic biomarkers for CRC.
• To develop a risk model for CRC diagnosis and prognosis.

Main Methods:

• Analysis of CRC datasets (TCGA-CRC, GSE17538) to identify differentially expressed and LDCD-related genes.
• Construction of a prognostic risk model using Cox regression and LASSO analysis.
• Evaluation of prognostic factors, tumor immune microenvironment, drug sensitivity, and gene expression in clinical samples.

Main Results:

• A total of 37 candidate genes were identified, with ATP6V0A4, CLU, and IL13RA2 selected for the risk model.
• The risk model demonstrated favorable diagnostic performance for CRC, incorporating risk score, age, and pathological T stage.
• High-risk group exhibited increased tumor immune dysfunction and exclusion; significant drug sensitivity differences were observed between risk subgroups.

Conclusions:

• ATP6V0A4, CLU, and IL13RA2 are identified as potential biomarkers associated with LDCD in CRC.
• The developed risk model shows promise for CRC diagnosis and prognosis.
• Findings provide insights for developing novel therapeutic strategies targeting LDCD in CRC.