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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

14.9K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Articles linked to this work by shared authors, journal, and citation graph.

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Volume electron microscopy reveals bacterial endosymbiosis within host mitochondria.

Communications biology·2026
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Isolation and Cultivation of Diplonemids.

Methods in molecular biology (Clifton, N.J.)·2026
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Preparation of Diplonemid Samples for Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
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The genome sequences of the diplonemid protist <i>Rhynchopus euleeides</i> YPF1915 and its bacterial endosymbiont <i>Candidatus</i> Syngnamydia salmonis (Chlamydiota).

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Description of new diplonemids (Diplonemea, Euglenozoa) and their endosymbionts: Charting the morphological diversity of these poorly known heterotrophic flagellates.

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Related Experiment Video

Updated: Jan 17, 2026

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
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Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

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A New Age of Advanced Volume Microscopy for Protists.

Daria Tashyreva1, Luca Cirino2, Noriko Okamoto3,4

  • 1Institute of Evolutionary Biology, Faculty of Biology, University of Warsaw, Warsaw, Poland.

The Journal of Eukaryotic Microbiology
|September 18, 2025
PubMed
Summary

Volume microscopy reveals cell 3D architecture, revolutionizing cell biology. This review guides protistology research using advanced imaging techniques for unexplored microbial eukaryotes.

Keywords:
FIB‐SEMSBF‐SEMcorrelative light electron microscopycryo‐electron microscopyelectron microscopyelectron tomographyexpansion microscopyprotistologyvolume microscopy

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Area of Science:

  • Cell Biology
  • Microscopy
  • Protistology

Background:

  • Volume microscopy is crucial for understanding cell 3D architecture.
  • Technological advances in light and electron microscopy have transformed cell biology.
  • Advanced microscopy techniques are increasingly accessible globally.

Purpose of the Study:

  • To review historical and emerging volume microscopy technologies.
  • To discuss the benefits and drawbacks of various imaging techniques.
  • To aid protistology in selecting appropriate methods for 3D investigations.

Main Methods:

  • Overview of volume microscopy techniques.
  • Analysis of imaging benefits and drawbacks.
  • Focus on applications in protistology.

Main Results:

  • Volume microscopy provides essential 3D cell architecture data.
  • Accessible advanced microscopy fuels biological discovery.
  • Protistology can benefit from detailed 3D structural insights.

Conclusions:

  • Volume microscopy is key to advancing protistology.
  • Informed technique selection is vital for 3D protist research.
  • Understanding 3D form and function is now more achievable.