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New compounds with anti-inflammatory activity from the roots of Isatis indigotica
Chen-Yu Jiang1, Jia-Tong Wu1, Juan Pan1
1Key Laboratory of Basic and Application Research of Beiyao, (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China.
Abstract:
Four new compounds, isatigotindoledioside G (1), methyl-9S,12S,13S-trihydroxyoctadeca-10E,15E-dienoate (2), 9S,12S,13S-trihydroxyoctadeca-10E,15E-dienoic acid (3), and one new artificial product ethyl 9S,12S,13S-trihydroxyoctadeca-10E,15Z-dienoate (4), along with fourteen recognized compounds (5-18), were extracted from the roots of Isatis indigotica. These compounds' structures were characterized using NMR spectroscopy, HR-ESI-MS, ECD calculations, and literature comparisons. Compounds 5-6 and 10-11 showed appreciable anti-inflammatory properties, and compounds 1-4, 12-13, and 17-18 showed mild anti-inflammatory properties when evaluated in the LPS-stimulated RAW264.7 cell model.
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