Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A culturally integrative occupational therapy practice model based on traditional Chinese health culture in Mainland China.

Hong Kong journal of occupational therapy : HKJOT·2025

Same author

Influence of COVID-19 public health restrictions on community-acquired pneumonia pathogens in children in Henan, China: a multicenter retrospective study.

BMC infectious diseases·2024

Same author

Timing of early correction of mandibular hypoplasia in skeletal class II malocclusion: a review.

The Journal of clinical pediatric dentistry·2023

Same author

Enhanced Efficacy of Gefitinib in Drug-Sensitive and Drug-Resistant Cancer Cell Lines after Arming with a Singlet Oxygen Releasing Moiety.

ChemMedChem·2020

Same author

Epidermal Growth Factor Receptor-Targeted Delivery of a Singlet-Oxygen Sensitizer with Thermal Controlled Release for Efficient Anticancer Therapy.

Molecular pharmaceutics·2019

Same author

Thioketal-crosslinked: ROS-degradable polycations for enhanced in vitro and in vivo gene delivery with self-diminished cytotoxicity.

Journal of biomaterials applications·2019

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

PACLseq: A Standalone Diagnostic Method for Ph-Like Acute Lymphoblastic Leukemia Using Nanopore Sequencing.

Hang Zhang¹, Huan Yu², Yanmei Chen²

¹Department of Hematology, Institute of Hematology West China Hospital of Sichuan University Chengdu Sichuan China.

|September 19, 2025

Summary

A new method, Partial Anchored Capture and Long-Read Sequencing (PACLseq), rapidly and accurately detects Philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL) fusion genes. This breakthrough improves diagnostic efficiency and patient outcomes for Ph-like ALL.

Keywords:

Philadelphia chromosome–like acute lymphoblastic leukemia Target transcriptome sequencing gene fusion long‐read sequencing

More Related Videos

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Related Experiment Videos

Last Updated: Jun 16, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Area of Science:

Genomics
Molecular Diagnostics
Oncology

Background:

Philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL) diagnosis relies on detecting specific fusion genes.
Current diagnostic methods for Ph-like ALL are time-consuming, costly, and struggle with degraded samples.

Purpose of the Study:

To develop and validate a rapid, cost-effective, and accurate method for detecting Ph-like ALL-related fusion genes.
To address the limitations of existing diagnostic workflows for Ph-like ALL.

Main Methods:

Introduction of Partial Anchored Capture and Long-Read Sequencing (PACLseq), a nanopore-sequencing-based approach.
Design of a targeted gene panel for Ph-like ALL, including key genes like ABL1, JAK2, and CRLF2.
Validation of PACLseq on 47 clinical samples, including degraded RNA specimens.

Main Results:

PACLseq demonstrated 93.3% sensitivity and 100% specificity in degraded RNA samples (RIN > 3).
The method successfully detected fusion genes in low-quality RNA samples (RIN ≤ 3) with fragmented transcripts.
PACLseq provides results in 3 days, a significant reduction from 7-14 days, with 50% cost savings.

Conclusions:

PACLseq offers a rapid, accurate, and cost-effective solution for Ph-like ALL fusion gene detection.
The technology enhances diagnostic efficiency, enabling timely treatment decisions for Ph-like ALL patients.
PACLseq has the potential to significantly improve patient management and outcomes in Ph-like ALL.