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Published on: April 25, 2018
LncRNA Xist acts as a miR-486-5p sponge to modulate myoblast proliferation by recruiting Stk4
Wenlun Wang1,2, Huang Yan3, Li Wenbin3
1Department of Food Science and Engineering, Moutai Institute, Renhuai,, 564507, Guizhou, China. 1019159335@qq.com.
Abstract:
Cell proliferation plays a crucial role in muscle atrophy-regeneration. However, the functional roles of Long non-coding RNA X-inactive specific transcript (lncRNA Xist) in C2C12 cell proliferation remain poorly characterized. We identified lncRNA Xist as a regulator of cell proliferation kinetics by acting as a competing endogenous RNA (ceRNA) in C2C12 cells. Downregulation or overexpression of lncRNA Xist correlated with enhanced or impaired cell proliferation, respectively. Mechanistically, lncRNA Xist sponges miR-486-5p to regulate serine/threonine kinase 4 (Stk4) expression, thereby modulating C2C12 cell proliferation in vitro. The lncRNA Xist/miR-486-5p/Stk4 ceRNA network plays an essential role in C2C12 proliferation, suggesting lncRNA Xist as a potential therapeutic target for muscle atrophy.
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